Calculate and fit MTT model from absorbance data

Usage

mtt_calc(x, ...)

# S3 method for data.frame
mtt_calc(x, ic_pct = 50, ...)

# S3 method for spectramax
mtt_calc(x, condition_names, drug_conc, ic_pct = 50, ...)

# S3 method for gp
mtt_calc(x, ic_pct = 50, ...)

Arguments

x: A data.frame, spectramax, or gp object. See Details.
...: Arguments passed to their respective methods
ic_pct: numeric. The %IC desired, where 25 would represent the concentration at which growth was reduced by 25% vs baseline
condition_names: What to name each 'sector' of the plate
drug_conc: a numeric vector containing drug concentrations of the conditions, from left to right

Value

a tibble

Details

If supplied with a data.frame, mtt will expect columns condition, drug, nm562, and nm660.

If supplied with a spectramax object, everything should be in place.

Using a gp object (NOT CURRENTLY SUPPORTED) is a good idea if you have a 'non-standard' plate layout (standard being each quarter of the plate is a condition)